Requesting and interpreting tests -- Disturbances of water, sodium and potassium balance -- Acid' base balance and oxygen transport -- Renal disease -- Disorders of calcium, phosphate and magnesium metabolism -- Diabetes mellitus and hypoglycaemia -- Disorders of the hypothalamus and pituitary -- Abnormalities of thyroid function -- Disorders of the adrenal cortex and medulla -- Investigation of gonadal function infertility, menstrual irregularities and hirsutism -- Pregnancy and antenatal screening -- Cardiovascular disorders -- Liver disease -- Gastrointestinal tract disease -- Nutrition -- Trauma, inflammation and immunity -- Malignancy and tumour markers -- Disorders of iron and porphyrin metabolism -- Uric acid, gout and purine metabolism -- Central nervous system and cerebrospinal fluid -- Therapeutic drug monitoring and chemical toxicology -- Clinical biochemistry in paediatrics and the elderly.